ASTM E578-2007 荧光测量系统的线性度用标准试验方法

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【英文标准名称】:StandardTestMethodforLinearityofFluorescenceMeasuringSystems
【原文标准名称】:荧光测量系统的线性度用标准试验方法
【标准号】:ASTME578-2007
【标准状态】:现行
【国别】:
【发布日期】:2007
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E13.01
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:分析方法;荧光;线性度;测量;光学;测试
【英文主题词】:fluorescencespectrometers;molecularluminescence;molecularspectroscopy
【摘要】:Therangeofconcentrationofafluorescingsubstanceinsolutionoverwhichthefluorescencevarieslinearlywiththeconcentrationistherangemostusefulforquantitativeanalysis.Thisrangeisaffectedbypropertiesofthesolutionunderanalysisandbyfeaturesofthemeasuringsystem.Thistestmethodprovidesameansoftestingtheperformanceofafluorescencemeasuringsystemandofdeterminingtheconcentrationrangeoverwhichthesystemissuitableformakingagivenquantitativeanalysis.Thistestmethodisnotmeantforcomparingtheperformanceofdifferentfluorescencemeasuringinstruments.1.1Thistestmethodcoversaprocedureforevaluatingthelimitsofthelinearityofresponsewithfluorescenceintensityoffluorescence-measuringsystemsunderoperatingconditions.Particularattentionisgiventoslitwidths,filters,andsamplecontainers.Thistestmethodcanbeusedtotesttheoveralllinearityunderawidevarietyofinstrumentalandsamplingconditions.Theresultsobtainedapplyonlytothetestedcombinationofslitwidthandfilters,andthesize,typeandilluminationofthesamplecuvette,allofwhichmustbestatedinthereport.Thesourcesofnonlinearitymaybethemeasuringelectronics,excessiveabsorptionofeithertheexcitingoremittedradiation,orboth,andthesamplehandlingtechnique,particularlyatlowconcentrations.1.2Thistestmethodhasbeenappliedtofluorescence-measuringsystemsutilizingcontinuousandlow-energyexcitationsources(forexample,anexcitationsourceof450-Welectricalinputorless).Thereisnoassurancethatextremelyintenseilluminationwillnotcausephotodecompositionofthecompoundssuggestedinthistestmethod.Forthisreasonitisrecommendedthatthistestmethodnotbeindiscriminatelyemployedwithhigh-intensitylightsources.Itisnotatestmethodtodeterminethelinearityofresponseofothermaterials.Ifthistestmethodisextendedtoemployotherchemicalsubstances,theprincipleswithincanbeapplied,butnewmaterialparameters,suchastheconcentrationrangeoflinearity,mustbeestablished.Theusershouldbeawareofthepossibilitythattheseothersubstancesmayundergodecomposition,oradsorptionontocontainers.1.3Thistestmethodhasbeenappliedtofluorescence-measuringsystemsutilizingasingledetector,thatis,aphotomultipliertubeorasinglephotodiode.Ithasnotbeendemonstratedifthismethodiseffectiveforphoto-arrayinstrumentssuchasthoseusingaCCDoradiodearraydetector.1.4Thistestmethodisapplicableto10-mmpathlengthcuvetteformatsandinstrumentscoveringawavelengthrangewithin190to900nm.Theuseofothersampleformatshasnotbeenestablishedwiththistestmethod.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:N34
【国际标准分类号】:17_180_30
【页数】:3P.;A4
【正文语种】: